Opened in June 2001, it is the result of exhibition systems that combine science and teaching, with a constant search for new incentives and languages, on the most current ecological themes. The rooms host realistic dioramas, faithful reconstructions of the most characteristic environments of the Prenestini Mountains such as pasture, the meadow, mixed wood and others, implemented by explanatory panels on the topics related to biology, geology and paleontology of the territory, as well as cutting-edge equipment for the observation and study of nature as microscopes and multimedia supports.
The museum, located inside the historic Palazzo Barberini, is divided into two main sections: - the reception area and bookshop, adjacent to the two "marvelous rooms", spaces dedicated to naturalistic themes and rich in materials to be observed, thanks to the scientific laboratory developed inside; - the museum area, consisting of two exhibition halls housed in the attic of the building in an evocative setting that catapults the visitor on a realistic journey to discover nature. The first room deals with the theme of biodiversity illustrating the different ecosystems in their original flora and fauna; the second room deals with the origins of the Monti Prenestini, deepening the themes of geology and palaeontology.
Inside the municipal park, the museum has recently set up an educational and laboratory area, a natural evolution of a continuous search for increasingly direct and stimulating languages: it is a fragment of anthropized green in which to rediscover situations and ideas that allow the user to interact with the environment, its components and its ecological dynamics through an unmediated relationship. The educational pond with its butterflies and water lilies, display boards containing traces and natural materials that can be touched with the hand and the "sensory garden" intended as a path of perfumes and colors to stimulate the five senses. Each route has been specially recreated to discover and assimilate the dynamics that underlie the most typical environments of the Monti Prenestini, taking into account the needs of each species and proposing a vegetational association as similar as possible to the spontaneous association.
The museum is free of architectural barriers and has an elevator, ramps and toilets suitable for people with mobility disabilities. The teaching garden is fully equipped, accessible to people with motor disabilities and equipped with subsidies useful for the involvement of users with mental and visual disabilities.